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Readcall · Sanger, PCR, arrays and parentage

From first primerto signed result.

Readcall runs genetic tests from assay design to signed result. Sanger, real-time and digital PCR, SNP arrays and parentage are available; your scientists review and sign. Every result carries its proof.

ITGA2B cats · well F5 · reverse read

Real public trace
Readcall's call
Clear C/C
Result
N/N · No copies of the ITGA2B insertion were found.
Read quality
Mean Q54, against the test's Q20
Analyst

Review by exception

Only the wells that need you.

On a public plate of 22 cats, Readcall confirmed 20 clear calls in one sweep and brought the cat it could not call from one read to a person.

20 clear calls, confirmed in one sweep2 wells brought to a person

ITGA2B cats · well G12Needs you

Needs a second read

The affected cat's read shows the insertion, g.506_507dup, in one read only. Readcall reports an insertion once a second read confirms it, so it never guesses.

Available now

Three phases, one recorded workflow.

The shared demo uses public and synthetic data. Automatic qPCR calling remains unavailable until a laboratory passes its own validation and concordance study.

  • Phase 1

    Sanger genotyping

    Import whole plates, review traces, check twice and release signed results.

  • Phase 2

    Molecular biology workbench

    Design and check primers, build constructs, confirm sequences and keep versioned projects.

  • Phase 4

    PCR, arrays and parentage

    Analyse real-time and digital PCR, import SNP arrays and release parentage results.

In development · Phase 3

NGS is not part of the current demo.

Run intake, mapping, calling and review are still being developed and validated. The separate NGS compute worker is not deployed on the shared demo server.

Design view of a planned NGS panel review card
Planned NGS review. This workflow is in development and is not available in the current demo.

Release

Signed, locked and checkable.

Release is held, not clicked. Results lock, each certificate carries a check code, and a later change becomes version 2.

Try the sample certificate's code.

Certificate · ITGA2B cats/F5/1

Version 1 · In force

Check code

245P-AT2X

Sample
6+ITGA2B_F
Test
ITGA2B insertion in cats, version 1
Result
N/N · No copies of the ITGA2B insertion were found.
Call
C/C · felCat9_chrE1_44415563_44416563:g.501=
Method
tracy 0.9.1, readcall rules 5, read quality 2
Reported by
Analyst 8, single analysis
Issued
4 October 2026

Records

Every action, on the record.

Who, when, the value before and after, and why. Each entry holds the hash of the one before, so an altered entry is found.

Plate record · ITGA2B cats

24 entries, none altered

  1. 01

    Imported

    sample sheet cat-sheet.csv, 22 wells, 23 files kept unchanged · Analyst 8, 12:43:23 UTC

    86d9239eb4after nothing

  2. 02

    Kept no call, back for sequencing · G12

    chrE1:44416063 C>CGG no call · Analyst 8, 12:45:26 UTC

    4b767bf484after 86d9239eb4

  3. 04

    Confirmed in the sweep · A5

    chrE1:44416063 C>CGG homozygous reference · Analyst 8, 12:45:26 UTC

    fab6285eebafter 9e67a55f94

  4. 24

    Released

    version 1, single analysis: 20 results locked, 2 back for sequencing, no controls · Analyst 8, 12:45:26 UTC

    962b848a41after 2384ca08bd

Accuracy

0wrong calls on public traces whose genotypes are known.

Published as we measure it. Engineering evidence for now: the validation pack, with its method and truth sets, is in progress.

How we measure
57
Correct callsCalled, and matching the genotype known from elsewhere
51
No callsBrought to a person, never into a result
108
Public targetsCalling rules 5, run on 3 October 2026

See Readcall with safe demo data.

Request an invite-only demonstration using public and synthetic data. Client and human samples do not go on the shared demo server.